mouse anti-human fgf2  (PeproTech)

Journal: Molecular Neurobiology

Article Title: Neuronal Stem Cells from Late-Onset Alzheimer Patients Show Altered Regulation of Sirtuin 1 Depending on Apolipoprotein E Indicating Disturbed Stem Cell Plasticity

doi: 10.1007/s12035-023-03633-z

Figure Lengend Snippet: List of primers for transcript analysis

Article Snippet: Mouse IgG anti-human FGF2 , IF: 1:100 , Bio-Techne , NBP1-47749.

Techniques:

Journal: Molecular Neurobiology

Article Title: Neuronal Stem Cells from Late-Onset Alzheimer Patients Show Altered Regulation of Sirtuin 1 Depending on Apolipoprotein E Indicating Disturbed Stem Cell Plasticity

doi: 10.1007/s12035-023-03633-z

Figure Lengend Snippet: List of antibodies for WB, IF, and flow cytometry analysis

Article Snippet: Mouse IgG anti-human FGF2 , IF: 1:100 , Bio-Techne , NBP1-47749.

Techniques: Flow Cytometry, Recombinant

Journal: Molecular Neurobiology

Article Title: Neuronal Stem Cells from Late-Onset Alzheimer Patients Show Altered Regulation of Sirtuin 1 Depending on Apolipoprotein E Indicating Disturbed Stem Cell Plasticity

doi: 10.1007/s12035-023-03633-z

Figure Lengend Snippet: Aging markers in NSCs are affected by late-onset AD. A Scheme illustrating the sample collection of NSCs from two iPSC clones of the same donor. Quantification of B transcript and D protein amounts of the aging markers APOE, ATG7, FGF2, p21, PTEN, SIRT1, and STAT3. Results are shown as mean ± SEM (* p = 0.05). The bar charts compare three healthy iPSCs (WISCi004-B, WAi001-B, and MLUi009-A) versus four AD iPSCs (MLUi007-H/J and MLUi008-B/F) on 7 d, which were differentiated three times into NSCs ( N = 3 differentiations; healthy: n = 9, AD: n = 12). E Representative image of WB membranes. C Telomere length measured by multiplex QRT-PCR in iPSCs on 0 d and in NSCs on 7 d, shown as the ratio NSCs/iPSCs grouped according to health status ( N = 3 differentiations; healthy: n = 9, AD: n = 12). Results are shown as mean ± SEM (* p = 0.05)

Article Snippet: Mouse IgG anti-human FGF2 , IF: 1:100 , Bio-Techne , NBP1-47749.

Techniques: Clone Assay, Multiplex Assay, Quantitative RT-PCR

Journal: Molecular Neurobiology

Article Title: Neuronal Stem Cells from Late-Onset Alzheimer Patients Show Altered Regulation of Sirtuin 1 Depending on Apolipoprotein E Indicating Disturbed Stem Cell Plasticity

doi: 10.1007/s12035-023-03633-z

Figure Lengend Snippet: Detection of marker proteins for cellular aging in NSCs through immunofluorescence (IF). IF analysis showed cellular localization of APOE, ATG7, FGF2, p21, PTEN, SIRT1, and STAT3. Protein of interest is shown in green versus DNA staining in blue (scale bar, 100 μm)

Article Snippet: Mouse IgG anti-human FGF2 , IF: 1:100 , Bio-Techne , NBP1-47749.

Techniques: Marker, Immunofluorescence, Staining

Journal: Molecular Neurobiology

Article Title: Neuronal Stem Cells from Late-Onset Alzheimer Patients Show Altered Regulation of Sirtuin 1 Depending on Apolipoprotein E Indicating Disturbed Stem Cell Plasticity

doi: 10.1007/s12035-023-03633-z

Figure Lengend Snippet: Aging markers in NSCs are affected by APOE genotype. A Transcript and B protein expression analysis of the aging markers APOE, ATG7, FGF2, p21, PTEN, SIRT1, and STAT3. Results are shown as mean ± SEM (* p = 0.05). The bar charts compare two iPSCs carrying APOE3 (WISCi004-B and MLUi009-A) versus five iPSCs carrying APOE4 (WAi001-B, MLUi007-H/J and MLUi008-B/F) on 7 d, which were differentiated three times into NSCs ( N = 3 differentiations; APOE3: n = 6, APOE4: n = 15). C Telomere length measured by multiplex QRT-PCR in iPSCs on 0 d and in NSCs on 7 d, shown as the ratio NSCs/iPSCs grouped according to APOE status ( N = 3 differentiations; APOE3: n = 6, APOE4: n = 15). Results are shown as mean ± SEM (* p = 0.05). D Manipulation of APOE gene expression by APOE siRNAs and APOE3 plasmids in NSCs generated from reference cell line WISCi004-B on 7 d. No morphological changes are shown by phase contrast imaging up to 4 d after transfection. WB analysis showed the strongest APOE protein repression by APOE siRNAs and strongest APOE induction by APOE3 plasmids on 4 d. Representative images of stained WB membranes are shown (scale bar, 100 μm). Transcript analysis of aging markers in NSCs on 4 d after E APOE inhibition and F APOE induction. Results are shown as mean ± SEM (* p = 0.05). For APOE inhibition, MLUi009-A (healthy matched control, APOE3 carrier) was differentiated into NSCs and transfected with siRNAs on 7 d ( N = 3 differentiations; mock: n = 3, siRNA: n = 3). For APOE induction, the MLUi007-H from (AD, APOE4 carrier) was differentiated into NSCs and transfected with APOE3 plasmids at 7 d ( N = 3 differentiations; mock: n = 3, APOE3 plasmids: n = 3)

Article Snippet: Mouse IgG anti-human FGF2 , IF: 1:100 , Bio-Techne , NBP1-47749.

Techniques: Expressing, Multiplex Assay, Quantitative RT-PCR, Generated, Imaging, Transfection, Staining, Inhibition

Journal: Molecular Neurobiology

Article Title: Neuronal Stem Cells from Late-Onset Alzheimer Patients Show Altered Regulation of Sirtuin 1 Depending on Apolipoprotein E Indicating Disturbed Stem Cell Plasticity

doi: 10.1007/s12035-023-03633-z

Figure Lengend Snippet: Significant mRNA expression changes observed in aging markers

Article Snippet: Mouse IgG anti-human FGF2 , IF: 1:100 , Bio-Techne , NBP1-47749.

Techniques: Expressing, Plasmid Preparation

Journal: Molecular Neurobiology

Article Title: Neuronal Stem Cells from Late-Onset Alzheimer Patients Show Altered Regulation of Sirtuin 1 Depending on Apolipoprotein E Indicating Disturbed Stem Cell Plasticity

doi: 10.1007/s12035-023-03633-z

Figure Lengend Snippet: Proposed mechanisms for APOE and its impact on aging markers for regulating NSC plasticity. Observed effects on PTEN, ATG7, FGF2, and SIRT1 may reflect the impact of APOE on RTK signaling including FGF2 as key signaling molecule, SIRT1 as transcriptional regulator, and PTEN and ATG7 as target genes. RTKs: receptor tyrosine kinases; JAKs: Janus kinases; AKT: protein kinases B; gray: Golgi apparatus; green arrows: APOE signaling; violet arrows: aging markers signaling via JAKs, RTKs, and Notch

Article Snippet: Mouse IgG anti-human FGF2 , IF: 1:100 , Bio-Techne , NBP1-47749.

Techniques:

Journal: Molecular Neurobiology

Article Title: Neuronal Stem Cells from Late-Onset Alzheimer Patients Show Altered Regulation of Sirtuin 1 Depending on Apolipoprotein E Indicating Disturbed Stem Cell Plasticity

doi: 10.1007/s12035-023-03633-z

Figure Lengend Snippet: List of primers for transcript analysis

Article Snippet: Mouse IgG anti-human FGF2 , WB: 1:100 , Santa Cruz , sc-136255.

Techniques:

Journal: Molecular Neurobiology

Article Title: Neuronal Stem Cells from Late-Onset Alzheimer Patients Show Altered Regulation of Sirtuin 1 Depending on Apolipoprotein E Indicating Disturbed Stem Cell Plasticity

doi: 10.1007/s12035-023-03633-z

Figure Lengend Snippet: List of antibodies for WB, IF, and flow cytometry analysis

Article Snippet: Mouse IgG anti-human FGF2 , WB: 1:100 , Santa Cruz , sc-136255.

Techniques: Flow Cytometry, Recombinant, Control

Journal: Molecular Neurobiology

Article Title: Neuronal Stem Cells from Late-Onset Alzheimer Patients Show Altered Regulation of Sirtuin 1 Depending on Apolipoprotein E Indicating Disturbed Stem Cell Plasticity

doi: 10.1007/s12035-023-03633-z

Figure Lengend Snippet: Aging markers in NSCs are affected by late-onset AD. A Scheme illustrating the sample collection of NSCs from two iPSC clones of the same donor. Quantification of B transcript and D protein amounts of the aging markers APOE, ATG7, FGF2, p21, PTEN, SIRT1, and STAT3. Results are shown as mean ± SEM (* p = 0.05). The bar charts compare three healthy iPSCs (WISCi004-B, WAi001-B, and MLUi009-A) versus four AD iPSCs (MLUi007-H/J and MLUi008-B/F) on 7 d, which were differentiated three times into NSCs ( N = 3 differentiations; healthy: n = 9, AD: n = 12). E Representative image of WB membranes. C Telomere length measured by multiplex QRT-PCR in iPSCs on 0 d and in NSCs on 7 d, shown as the ratio NSCs/iPSCs grouped according to health status ( N = 3 differentiations; healthy: n = 9, AD: n = 12). Results are shown as mean ± SEM (* p = 0.05)

Article Snippet: Mouse IgG anti-human FGF2 , WB: 1:100 , Santa Cruz , sc-136255.

Techniques: Clone Assay, Multiplex Assay, Quantitative RT-PCR

Journal: Molecular Neurobiology

Article Title: Neuronal Stem Cells from Late-Onset Alzheimer Patients Show Altered Regulation of Sirtuin 1 Depending on Apolipoprotein E Indicating Disturbed Stem Cell Plasticity

doi: 10.1007/s12035-023-03633-z

Figure Lengend Snippet: Detection of marker proteins for cellular aging in NSCs through immunofluorescence (IF). IF analysis showed cellular localization of APOE, ATG7, FGF2, p21, PTEN, SIRT1, and STAT3. Protein of interest is shown in green versus DNA staining in blue (scale bar, 100 μm)

Article Snippet: Mouse IgG anti-human FGF2 , WB: 1:100 , Santa Cruz , sc-136255.

Techniques: Marker, Immunofluorescence, Staining

Journal: Molecular Neurobiology

Article Title: Neuronal Stem Cells from Late-Onset Alzheimer Patients Show Altered Regulation of Sirtuin 1 Depending on Apolipoprotein E Indicating Disturbed Stem Cell Plasticity

doi: 10.1007/s12035-023-03633-z

Figure Lengend Snippet: Aging markers in NSCs are affected by APOE genotype. A Transcript and B protein expression analysis of the aging markers APOE, ATG7, FGF2, p21, PTEN, SIRT1, and STAT3. Results are shown as mean ± SEM (* p = 0.05). The bar charts compare two iPSCs carrying APOE3 (WISCi004-B and MLUi009-A) versus five iPSCs carrying APOE4 (WAi001-B, MLUi007-H/J and MLUi008-B/F) on 7 d, which were differentiated three times into NSCs ( N = 3 differentiations; APOE3: n = 6, APOE4: n = 15). C Telomere length measured by multiplex QRT-PCR in iPSCs on 0 d and in NSCs on 7 d, shown as the ratio NSCs/iPSCs grouped according to APOE status ( N = 3 differentiations; APOE3: n = 6, APOE4: n = 15). Results are shown as mean ± SEM (* p = 0.05). D Manipulation of APOE gene expression by APOE siRNAs and APOE3 plasmids in NSCs generated from reference cell line WISCi004-B on 7 d. No morphological changes are shown by phase contrast imaging up to 4 d after transfection. WB analysis showed the strongest APOE protein repression by APOE siRNAs and strongest APOE induction by APOE3 plasmids on 4 d. Representative images of stained WB membranes are shown (scale bar, 100 μm). Transcript analysis of aging markers in NSCs on 4 d after E APOE inhibition and F APOE induction. Results are shown as mean ± SEM (* p = 0.05). For APOE inhibition, MLUi009-A (healthy matched control, APOE3 carrier) was differentiated into NSCs and transfected with siRNAs on 7 d ( N = 3 differentiations; mock: n = 3, siRNA: n = 3). For APOE induction, the MLUi007-H from (AD, APOE4 carrier) was differentiated into NSCs and transfected with APOE3 plasmids at 7 d ( N = 3 differentiations; mock: n = 3, APOE3 plasmids: n = 3)

Article Snippet: Mouse IgG anti-human FGF2 , WB: 1:100 , Santa Cruz , sc-136255.

Techniques: Expressing, Multiplex Assay, Quantitative RT-PCR, Gene Expression, Generated, Imaging, Transfection, Staining, Inhibition, Control

Journal: Molecular Neurobiology

Article Title: Neuronal Stem Cells from Late-Onset Alzheimer Patients Show Altered Regulation of Sirtuin 1 Depending on Apolipoprotein E Indicating Disturbed Stem Cell Plasticity

doi: 10.1007/s12035-023-03633-z

Figure Lengend Snippet: Significant mRNA expression changes observed in aging markers

Article Snippet: Mouse IgG anti-human FGF2 , WB: 1:100 , Santa Cruz , sc-136255.

Techniques: Expressing, Plasmid Preparation

Journal: Molecular Neurobiology

Article Title: Neuronal Stem Cells from Late-Onset Alzheimer Patients Show Altered Regulation of Sirtuin 1 Depending on Apolipoprotein E Indicating Disturbed Stem Cell Plasticity

doi: 10.1007/s12035-023-03633-z

Figure Lengend Snippet: Proposed mechanisms for APOE and its impact on aging markers for regulating NSC plasticity. Observed effects on PTEN, ATG7, FGF2, and SIRT1 may reflect the impact of APOE on RTK signaling including FGF2 as key signaling molecule, SIRT1 as transcriptional regulator, and PTEN and ATG7 as target genes. RTKs: receptor tyrosine kinases; JAKs: Janus kinases; AKT: protein kinases B; gray: Golgi apparatus; green arrows: APOE signaling; violet arrows: aging markers signaling via JAKs, RTKs, and Notch

Article Snippet: Mouse IgG anti-human FGF2 , WB: 1:100 , Santa Cruz , sc-136255.

Techniques:

Journal: Molecular neurobiology

Article Title: Neuronal Stem Cells from Late-Onset Alzheimer Patients Show Altered Regulation of Sirtuin 1 Depending on Apolipoprotein E Indicating Disturbed Stem Cell Plasticity.

doi: 10.1007/s12035-023-03633-z

Figure Lengend Snippet: Fig. 4 Detection of marker proteins for cellular aging in NSCs through immunofluorescence (IF). IF analysis showed cellular localization of APOE, ATG7, FGF2, p21, PTEN, SIRT1, and STAT3. Protein of interest is shown in green versus DNA staining in blue (scale bar, 100 μm)

Article Snippet: 1 3 Mouse IgG anti-human ACTB WB: 1:40,000 Merck A5441 Mouse IgG anti-human APOE WB: 1:500; IF: 1:500 Bio-Techne NB110-60531 Mouse IgG anti-human ATG7 WB: 1:1000; IF: 1:100 Bio-Techne MAB6608 Mouse IgG anti-human COL1A1 IF: 1:100 Merck C2456 Rabbit IgG anti-human CDH1 IF: 1:100 Abcam Ab40772 Mouse IgG anti-human FGF2 WB: 1:100 Santa Cruz sc-136255 Mouse IgG anti-human FGF2 IF: 1:100 Bio-Techne NBP1-47749 Rabbit IgG anti-human MSI1 IF: 1:100 Merck AB5977 Mouse IgG anti-human NES IF: 1:100 Santa Cruz Sc-23927 Rabbit IgG anti-human NEUROG3 IF: 1:100 Abcam Ab38548 Mouse IgG anti-human OCT4 (POU5F1) IF: 1:100 Santa Cruz Sc-5279 Mouse IgG anti-human PAX6 IF: 1:100 Santa Cruz Sc-53108 Goat IgG anti-human PRRX1 IF: 1:100 Bio-Techne NBP1-06067 Rabbit IgG anti-human p21m (CDKN1A) WB: 1:1000; IF: 1:800 Cell Signaling Technology 2947 Mouse IgG anti-human p16 (CDKN2A) IF: 1:100 Origene TA500036 Mouse IgM anti-human PTEN WB: 1:100; IF: 1:200 Thermo Fisher Scientific MA5-12278 Mouse IgG anti-human SIRT1 WB: 1:1000; IF: 1:1000 Bio-Techne NBP1-51641 Goat IgG anti-human SOX2 IF: 1:100 Santa Cruz Sc17320 Mouse IgG anti-human SOX17 IF: 1:100 Santa Cruz Sc-130295 Mouse IgM anti-human SSEA1 IF: 1:100 Santa Cruz Sc-21702 Mouse IgG anti-human STAT3 WB: 1:5000; IF: 1:100 Thermo Fisher Scientific MA1-13042 Mouse IgG anti-human VIM IF: 1:100 Merck MAB3400 Goat IgG anti-mouse HRP WB: 1:10,000 Dianova 115-035-003 Goat IgG anti-rabbit HRP WB: 1:3000 Cell Signaling Technology 7074 Goat IgM anti-mouse HRP WB: 1:4000 Thermo Fisher Scientific 62-6820 Donkey IgG anti-goat Alexa FluorTM 488 IF: 1:400 Thermo Fisher Scientific A11055 Goat IgG anti-mouse Alexa FluorTM 488 IF: 1:400 Thermo Fisher Scientific A11001 1 3

Techniques: Marker, Immunofluorescence, Staining

Journal: Molecular neurobiology

Article Title: Neuronal Stem Cells from Late-Onset Alzheimer Patients Show Altered Regulation of Sirtuin 1 Depending on Apolipoprotein E Indicating Disturbed Stem Cell Plasticity.

doi: 10.1007/s12035-023-03633-z

Figure Lengend Snippet: Fig. 6 Proposed mechanisms for APOE and its impact on aging markers for regulating NSC plasticity. Observed effects on PTEN, ATG7, FGF2, and SIRT1 may reflect the impact of APOE on RTK signaling including FGF2 as key signaling molecule, SIRT1 as transcrip- tional regulator, and PTEN and ATG7 as target genes. RTKs: receptor tyrosine kinases; JAKs: Janus kinases; AKT: protein kinases B; gray: Golgi apparatus; green arrows: APOE signaling; violet arrows: aging markers signaling via JAKs, RTKs, and Notch

Article Snippet: 1 3 Mouse IgG anti-human ACTB WB: 1:40,000 Merck A5441 Mouse IgG anti-human APOE WB: 1:500; IF: 1:500 Bio-Techne NB110-60531 Mouse IgG anti-human ATG7 WB: 1:1000; IF: 1:100 Bio-Techne MAB6608 Mouse IgG anti-human COL1A1 IF: 1:100 Merck C2456 Rabbit IgG anti-human CDH1 IF: 1:100 Abcam Ab40772 Mouse IgG anti-human FGF2 WB: 1:100 Santa Cruz sc-136255 Mouse IgG anti-human FGF2 IF: 1:100 Bio-Techne NBP1-47749 Rabbit IgG anti-human MSI1 IF: 1:100 Merck AB5977 Mouse IgG anti-human NES IF: 1:100 Santa Cruz Sc-23927 Rabbit IgG anti-human NEUROG3 IF: 1:100 Abcam Ab38548 Mouse IgG anti-human OCT4 (POU5F1) IF: 1:100 Santa Cruz Sc-5279 Mouse IgG anti-human PAX6 IF: 1:100 Santa Cruz Sc-53108 Goat IgG anti-human PRRX1 IF: 1:100 Bio-Techne NBP1-06067 Rabbit IgG anti-human p21m (CDKN1A) WB: 1:1000; IF: 1:800 Cell Signaling Technology 2947 Mouse IgG anti-human p16 (CDKN2A) IF: 1:100 Origene TA500036 Mouse IgM anti-human PTEN WB: 1:100; IF: 1:200 Thermo Fisher Scientific MA5-12278 Mouse IgG anti-human SIRT1 WB: 1:1000; IF: 1:1000 Bio-Techne NBP1-51641 Goat IgG anti-human SOX2 IF: 1:100 Santa Cruz Sc17320 Mouse IgG anti-human SOX17 IF: 1:100 Santa Cruz Sc-130295 Mouse IgM anti-human SSEA1 IF: 1:100 Santa Cruz Sc-21702 Mouse IgG anti-human STAT3 WB: 1:5000; IF: 1:100 Thermo Fisher Scientific MA1-13042 Mouse IgG anti-human VIM IF: 1:100 Merck MAB3400 Goat IgG anti-mouse HRP WB: 1:10,000 Dianova 115-035-003 Goat IgG anti-rabbit HRP WB: 1:3000 Cell Signaling Technology 7074 Goat IgM anti-mouse HRP WB: 1:4000 Thermo Fisher Scientific 62-6820 Donkey IgG anti-goat Alexa FluorTM 488 IF: 1:400 Thermo Fisher Scientific A11055 Goat IgG anti-mouse Alexa FluorTM 488 IF: 1:400 Thermo Fisher Scientific A11001 1 3

Techniques: